|
KMID : 0861020210360010067
|
|
Korea Journal of Herbology
2021 Volume.36 No. 1 p.67 ~ p.76
|
|
|
Development of tablets and evaluation of ingredient content and pharmacological effects of Yukgunja-tang
|
|
Kim Myoung-Jin
Choi Hye-Min
Yu Byung-Woo
|
|
|
Abstract
|
|
|
Objective : Yukgunja-tang is one of the herbal prescriptions widely used for functional indigestion. In this study, we evaluated the pharmacological effect through the Yukgunja-tang formulation development.
Methods : The RAW 264.7 cells were pretreated with Yukgunja-tang tablet (YGJT-T : 50, 100 and 200 ㎍/㎖) and then stimulated with lipopolysaccharide (LPS : 500 ng/㎖). Cell viability, inflammatory mediators such as nitric oxide (NO) and prostaglandin E2 (PGE2) and inflammatory cytokines (IL-1β, IL-6, and TNF-α) were measured. Also, ICR mice induced acute gastritis by oral administration of 150 mM HCl in 60% ethanol. The YGJT-T (30 ㎎/㎏) was pretreated for 3 days, and 150 mM HCl in 60% ethanol was orally administered 1 hour after the last drug treatment.
Mice were sacrificed 1 hour after oral administration of 150 mM HCl in 60% ethanol. The gastric mucosa was observed, and inflammatory cytokines in the gastric tissue were measured.
Results : The marker components of YGJT-T were determined by simultaneous analysis using HPLC. In RAW 264.7 cells, pretreatment of YGJT-T was non-toxic and inhibited the production of inflammatory mediators such as NO and PGE2 and suppressed inflammatory cytokines. In addition, pretreatment of YGJT-T improved bleeding and edema due to gastric lesions caused by acute gastritis and suppressed inflammatory cytokines.
Conclusion : In summary, our results confirmed that treatment with YGJT-T has anti-inflammatory and anti-gastritis effects in vitro and in vivo. Therefore, in this study, YGJT-T could support a potential strategy for the prevention and treatment of gastritis.
|
|
|
KEYWORD
|
|
|
Yukgunja-tang tablet (YGJT-T), lipopolysaccharide (LPS), pro-inflammatory cytokines, acute gastritis, anti-inflammatory, anti-gastritis
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|